谱析（上海）生物科技有限公司提供——25-OH Vitamin D2/D3 in Plasma and Serum by UHPLC 质谱诊断方案

一、说明
25-OH Vitamins D (D2, D3) are important parameters for the assessment of the vitamin D status. In addition to RECIPE's HPLC complete kit, a ClinRep@ Complete Kit for UHPLC systems is available. The UHPLC Complete Kit features a significantly increased sample throughput with reduced buffer consumption due to the very short chromatography duration of only 2 minutes. For system check, calibration and internal quality control a ClinTest@ Solution, a ClinCal@ Serum Calibrator (traceable to NIST standard SRM 972) and ClinChek@ Serum Controls are available.

二、产品优势
（1）UHPLC Kit for Vitamin D
（2）Injection interval: 2 min
（3）Traceable to NIST SRM 972

三、测试数据
Linearity:                  4.6 - 500 μg/I (D2)
3.7 - 500 μg/I (D3)
Recovery:                   99 - 104 %
LOD:                        2.8 μg/I (D2)
2.2 μg/I (D3)
LLOQ:                       4.6 μg/I (D2)
                            3.7 μg/I (D3)
Intraassay precision:      4.3 % (D2)
                            4.6 % (D3)
Interassay precision:       5.9 % (D2) 
                            3.9 % (D3) 

四、高效液相色谱参数
Pump:                       isocratic pump, flow rate: 0.75 ml/min
Injection volume:           5 μI
Injection interval:         2 min
UV-Detector:                264 nm
HPLC-Thermostat:            40°C 

五、样品制备



六、订购信息


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