谱析（上海）生物科技有限公司提供——Vitamin A and E in Serum/Plasma by UHPLC  质谱诊断方案

一、说明
Vitamin A (Retinol) und vitamin E (Tocopherol) belong to the fat soluble class of vitamins. In contrast to the water soluble an excessive administration may lead to hypervitaminosis. Vitamin A deficiency is the leading cause of preventable blindness in children and raises the risk of disease and death from severe infections. Vitamin E is an important antioxidant and is thought to have a role in preventing atherosclerosis by inhibiting oxidation of LDL. This ClinRep@ Complete Kit is designed for analysis on UHPLC systems. RECIPE's analytical method enables a high sample throughput by combining fast chromatography (1.2 min injection interval) and fast sample preparation (one step matrix precipitation). The analysis can be performed from serum or plasma. For quality assurance, reference materials with excellent stability are available.

二、产品优势
（1）Fast and simple 
（2）Injection interval: 1.2 min

三、测试数据
Linearity:                  Vitamin A:      0.04 - 3.96 mg/L
                  Vitamin E:      1.02 - 104 mg/L
Recovery:                   96- 109 %
LOD:                        Vitamin A:     0.013 mg/L
                            Vitamin E:     0.535 mg/L
LLOQ:                       Vitamin A:      0.04 mg/L
                            Vitamin E:     1.07 mg/L
Intraassay :               Vitamin A:     1.7-7.1 %
                            Vitamin E:     1.4-4.0 %
Interassay :                Vitamin A:     1.9-3.2 % 
                            Vitamin E:      1.4-2.3 % 







四、高效液相色谱参数
Pump:                       isocratic pump, flow rate: 0.8 ml/min
Injection volume:           5 μI
Injection interval:         1.2 min
UV-Detector:                325/295 nm
Column heater:              30 °C

五、样品制备



六、订购信息


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