谱析（上海）生物科技有限公司提供——Hemoglobin Variants and ß-Thalassemia Screening in Whole Blood 质谱诊断方案

一、说明
Defects in genes, which are responsible for the synthesis of the hemoglobin molecule, result in a group of hereditary diseases termed as "hemoglobinopathies". Depending on the molecular defect, hemoglobinopathies may be classified in "structural hemoglobin variants" and in "thalassemias". This test kit allows a reliable separation and identification of the common and clinically significant hemoglobin variants. For B-thalassemia screening the kit ensures an exact quantification of HbF and HbA2. A ready for use test solution (containing: HbA1c, HbF, HbAO, HbE, HbA2, HbD, Hbs, HbC), a hemolysate calibrator (containing: HbF, HbA2) as well as hemolysate controls in two levels of concentration are available for quality assurance.

二、产品优势
（1）可靠地分离和定量HbA2和HbF
（2）Hbs、HbC、HbE和HbD的安全识别
（3）可提供测试溶液，校准器和控制材料

三、测试数据
Intraassay precision:      1.0 - 3.3 % (HbF)
0.6 - 3.6 % (HbA2)
Interassay precision:       2.1 - 2.8% (HbF)     
0.7 - 2.4 (HbA2)
Dependence on total hemoglobin:
The HbF and HbA2 values are independent on the amount of total hemoglobin in a range from 6-24 g/dl total hemoglobin. 

四、高效液相色谱参数
Pump:                       binary gradient pump
Flow rate:                  1.2 ml/min
Injection volume:           20 μl
Injection interval:         8 min
UV/VIS-Detector:            415 nm
HPLC-Thermostat:            23 °C

五、样品制备



六、订购信息


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